Heterocyclic compounds

ABSTRACT

Heterocyclic compounds of the general formula: ##STR1## wherein, Z represents methylene group or sulfur atom, R represents a general formula: 
     
         G--E--D--B--A--.

This is a divisional of application Ser. No. 08/008,365, filed Jan. 22, 1993, which is a division application of Ser. No. 07/700,299, filed May 15, 1991, (U.S. Pat. No. 5,212,191), which is a division application of Ser. No. 333,227, filed on Apr. 5, 1989 (U.S. Pat. No. 5,053,414).

SUMMARY

The present invention is related to novel compounds having an inhibitory activity on prolyl endopeptidase.

More particularly, this invention is related to

1) novel heterocyclic compounds having an inhibitory activity on prolyl endopeptidase, of the following general formula: ##STR2## wherein all of the symbols have the same meaning as hereafter defined. 2) processes for the preparation of them, and

3) anti-amnesia agents containing them as an active ingredient.

BACKGROUND

Recent advance in neuroscience is making clear the natural shape of neurotransmitter substances deeply related to memory in the brain. It is said that some of these substances are neuropeptides containing prolines.

Recovery of the memory was reported by the dosing neuropeptide containing proline to experimental amnesia rats (See Science 211, 601 (1981)).

On the other hand, it is presumed that these neuropeptide hormones shall be metabolized by cerebral endogenous peptidases. Especially, prolyl endopeptidase (EC, 3. 4. 21. 26) might take part in these metabolisms closely (See J. Biochem., 94, 1179 (1983)).

From these facts, studies were in progress that it should be possible to prevent or treat amnesia by inhibiting prolyl endopeptidase and suppressing the metabolism of neurotransmitters. (See Protein, Nucleic acid and Enzyme 25(6), 513(1980); Nippon Nougei Kagaku Kaishi 58(11), 1147(1984); J. Neurochem., 41, 69(1983); ibid 42, 237(1984).)

For the purpose described above, several compounds were synthesized. For example, it is clear that N-benzyloxycarbonyl-glycyl-L-prolyl-chloromethane, N-benzyloxycarbonyl-L-prolyl-prolinal strongly inhibit prolyl endopeptidase (See J. Neurochem., 41, 69 (1983)). More recently, it is disclosed that the compounds shown below are effective for the above purpose.

(i) Pyrrolidine derivatives of the general formula: ##STR3## wherein, R¹ represents ##STR4## R² represents lower alkyl group, phenyl group which is unsubstituted or substituted or --CH₂ --R²².

With the proviso that, R³, R⁴, R⁵ independently represent hydrogen atom, halogen atom, hydroxy group, lower alkyl group, phenyl group which is unsubstituted or substituted,

--CH₂ R⁹, --X--R¹⁰, --O--CO--R¹², --NH₂, --NHR¹⁴

or NR¹⁷ R¹⁸, with the proviso that R⁹ represents phenyl group which is unsubstituted or substituted. X represents an oxygen atom or sulfur atom. R¹⁰ represents lower alkyl group, phenyl group which is substituted or unsubstituted or --CH₂ R¹¹, with the proviso that R¹¹ represents phenyl group which is unsubstituted or substituted. R¹² represents lower alkyl group, phenyl group which is unsubstituted or substituted or --CH₂ R¹³, with the proviso that R¹³ represents phenyl group which is unsubstituted or substituted. R¹⁵ represents lower alkyl group, cycloalkyl group of 5 or 6 ring members, phenyl group which is unsubstituted or substituted, --CH₂ R¹⁵, with the proviso that R¹⁵ represents phenyl group unsubstituted or substituted, or --CO--R¹⁶ with the proviso that R¹⁶ represents lower alkyl group or phenyl group which is unsubstituted or substituted. R¹⁷ and R¹⁸ independently represent lower alkyl group or --CH₂ R¹⁹ with the proviso that R¹⁹ represents phenyl group which is unsubstituted or substituted.

R⁶ represents a hydrogen atom or lower alkyl group. R⁷ represents a hydrogen atom, lower alkyl group, benzyl group, CH₃ --CH(OH)-- or --(CH₂)_(n) --R²⁰, with the proviso that n represents an integer of from 0 to 4. R²⁰ represents --OH, --SH, --NH₂, --SCH₃, ##STR5## --CONH₂, --NH--C(═NH)--NH₂, --CO₂ R²¹, with the proviso that R²¹ represents hydrogen atom, lower alkyl group or benzyl group, or heterocyclic ring group. R⁸ represents hydrogen atom or 5-membered heterocyclic ring group together with R⁷ and nitrogen atom, and carbon atom which are neighboured. R²² represents phenyl group which is unsubstituted or substituted.). See Japanese Patent Kokai No. 62-221666, i.e. European Patent Publication No. 238319.

ii) Pyrrolidine derivatives of its general formula: ##STR6## wherein R¹ represents ##STR7## R² represents ##EQU1## with the proviso that, R³, R⁴ and R⁵ independently represent hydrogen atom, halogen atom, hydroxy group, lower alkyl group, phenyl group which is unsubstituted or substituted, --CH₂ R⁹, --X--R¹⁰, --O--CO--R¹², --NH₂, --NHR¹⁴ or --NR¹⁷ R¹⁸, with the proviso that R⁹ represents phenyl group which is unsubstituted or substituted. X represents an oxygen atom or sulfur atom. R¹⁰ represents lower alkyl group, phenyl group which is unsubstituted or substituted, or --CH₂ R¹¹, with the proviso that R¹¹ represents phenyl group which is unsubstituted or substituted.) R¹² represents loweralkyl group, phenyl group which is unsubstituted or substituted or --CH₂ R¹³, with the proviso that R¹³ represents phenyl group which is unsubstituted or substituted. R¹⁴ represents lower alkyl group, cycloalkyl group of 5 or 6 rings members, phenyl group which is unsubstituted or substituted, --CH₂ R¹⁵, with the proviso that R¹⁵ represents phenyl group which is unsubstituted or substituted, or --CO--R¹⁶, with the proviso that R¹⁶ represents lower alkyl group, or phenyl group which is unsubstituted or substituted.) R¹⁷ and R¹⁸ independently represent lower alkyl group or --CH₂ R¹⁹, with the proviso that R¹⁹ represents phenyl group which is unsubstituted or substituted.

R⁶ represents hydrogen atom or lower alkyl group. R⁷ represents hydrogen atom, lower alkyl group, benzyl group, CH₃ --CH(OH)-- or --(CH₂)_(n) --R²⁰ with the proviso that, n represents an integer of from 0 to 4. R²⁰ represents --OH, --SH, --NH₂, --SCH₃, ##STR8## --CONH₂, --NH--C(═NH)--NH₂, --CO₂ R²¹, with the proviso that R²¹ represents hydrogen atom, lower alkyl group or benzyl group, or heterocyclic ring group. R⁸ represents hydrogen atom, or 5-membered heterocyclic ring group together with R⁷ and a nitrogen atom and carbon atom which are neighboured. R²² represents hydrogen atom, lower alkyl group, phenyl group which is unsubstituted or substituted, or --CH₂ R²⁴, with the proviso that R²⁴ represents phenyl group which is unsubstituted or substituted. R²³ represents lower alkyl group or when R²³ and R²³ are each taken together, they represent lower alkylene group. See Japanese Patent Kokai No. 62-221667, i.e. European Patent Publication No. 238319.

The present inventors have also filed an application related to prolinal derivatives having an activity of anti-amnesia, in advance of the present application, i.e.

(iii) Prolinal derivatives of general formula: ##STR9## wherein A represents alkylene or alkenylene group of from 1 to 8 carbon atom(s) or a saturated hydrocarbon ring of from 3 to 7 carbon atoms, R represents hydrogen atom, phenyl group, benzyl group, alkyl group of from 1 to 8 carbon atom(s) or cycloalkyl group of from 3 to 7 carbon atoms,

B represents alkylene group of from 1 to 8 atom(s) unsubstituted or substituted by phenyl group or benzyl group or a single bond,

D represents carbocyclic or heterocyclic ring unsubstituted or substituted by from one to three of halogen atom, alkyl or alkoxy group of from 1 to 4 carbon atom(s), nitro group or trifluoromethyl group. See Japanese Patent Application No. 62-290631, i.e. European Patent Publication No. 268190.

COMPARISON WITH THE PRIOR ART

The compounds of the present invention of the general formula (I) are novel compounds which have new modifications.

More concretely, each groups of the compounds of the general formula from (Ic) to (If) shown below which correspond to the group R of the compounds of the general formula (I) are new substituents. It was first found that the compounds of the general formula from (Ic) to (If) possess an inhibitory activity on prolyl endopeptidase by the present inventors.

The compounds of the general formula (Ia) and (Ib) are also novel.

The present inventors tried to modify the group D of the compounds of the general formula (C) in our application described hereinbefore. We confirmed that the compounds wherein the benzene ring was converted to another ring (including heterocyclic rings, fused rings, e.g. naphthalene, fluorence, furan etc.) also have the inhibitory activity on prolyl endopeptidase.

As the group G in the general formula (I) corresponds to the group D of the general formula (C), it is not difficult to expect that the compounds wherein the benzene ring as the group G was replaced by another ring will possess the inhibitory activity on prolyl endopeptidase, when the compounds of the present invention wherein the group G is a benzene ring possess the inhibitory activity.

DISCLOSURE OF THE INVENTION

The present invention is related to:

1) A heterocyclic (pyrrolidine derivative or thiazolidine derivative) compound of the general formula: ##STR10## wherein, Z represents methylene group or sulfur atom,

R represents the general formula:

    G--E--D--B--A--

wherein, A represents a bond, alkylene group of from 1 to 6 carbon atom(s), alkenylene group of from 2 to 6 carbon atoms, a group of the general formula: ##STR11## wherein Y represents alkylene group of from 1 to 4 carbon atom(s) or alkenylene group of from 2 to 4 carton atoms or a saturated hydrocarbon ring of from 4 to 7 carbon atoms or heterocyclic mono ring.

B represents a bond or alkylene group of from 1 to 6 carbon atom(s).

D represents a bond, oxygen atom, carbonyl group or a group of general formula:

    --NR.sup.1 --CO-- or --CO--NR.sup.1 --

wherein R¹ represents hydrogen atom, alkyl group of from 1 to 6 carbon atom(s), phenyl group or benzyl group.

E represents a bond, alkylene group of from 1 to 8 carbon atom(s) or alkylene group of from 1 to 8 carbon atom(s) substituted by phenyl or benzyl group.

G represents a carbocyclic or heterocyclic ring which is unsubstituted or substituted by 1˜3 of alkyl group of from 1 to 6 carbon atom(s), alkoxy group of from 1 to 6 carbon atom(s), halogen atom, trifluoromethyl group or nitro group.

L represents a group of the general formula:

    --CO--COR.sup.2

    --CH(OH)--COR.sup.2

    --CH(OR.sup.3).sub.2 ##STR12##

    --CH(OH)--R.sup.4

    --CO--R.sup.4

    --CO--CH.sub.2 --COR.sup.2

    --CH(OH)--CH.sub.2 --COR.sup.2

    --CO--CF.sub.2 --COR.sup.2

    --CH(OH)--CF.sub.2 --COR.sup.2

    --CO--CO--NR.sup.5 R.sup.6

or

    --CO--CH.sub.2 --CO--NR.sup.5 R.sup.6

wherein,

R² represents hydrogen atom, hydroxy group, alkyl group of from 1 to 6 carbon atom(s), alkoxy group of from 1 to 6 carbon atom(s), phenyl group, alkyl group of from 1 to 6 carbon atom(s) substituted by phenyl group or alkoxy group of from 1 to 6 carbon atom(s) substituted by phenyl group.

R³ represents alkyl group of from 1 to 6 carbon atom(s), phenyl group, or alkyl group of from 1 to 6 carbon atom(s) substituted by phenyl group.

R⁴ represents alkyl group of from 1 to 6 carbon atom(s), phenyl group, alkyl group of from 1 to 6 carbon atom(s) substituted by phenyl group or trifluoromethyl group.

R⁵ and R⁶ independently represents hydrogen atom, alkyl group of from 1 to 6 carbon atom(s), phenyl group, or alkyl group of from 1 to 6 carbon atom(s) substituted by phenyl group.

With the proviso that the following compounds are excluded:

(i) Compounds wherein both of A and B are bonds.

(ii) Compounds wherein Z is methylene group, and L is a group of general formula:

    --CO--COR.sup.2

    --CH(OH)--COR.sup.2

    --CH(OR.sup.3).sub.2 ##STR13## or

    --CH(OH)--R.sup.4

wherein all of the symbols are the same meaning as in hereinbefore defined.

and

R is

(1) a group of general formula:

    G'--E--CONH--B--A--

(2) a group of general formula:

    G'--CO--B--A--

(3) a group of general formula: ##STR14## (4) a group of general formula:

    G'--O--Alk--

(5) a group of general formula: ##STR15## (6) a group of general formula:

    G'--Alk--

(7) a group of general formula: ##STR16## or (8) a group of general formula: ##STR17## wherein, G' represents benzene ring which is unsubstituted or substituted by substituent(s).

Alk represents a bond or alkylene group of from 1 to 3 carbon atom(s).

p represents an integer of from 1˜5

and non-toxic salts or hydrate thereof

2) Processes for the preparation of them; and

3) Anti-amnesia agents containing them as an active ingredient.

The following groups of compounds are included in the present invention, that is: ##STR18## wherein, L¹ represents a group of general formula:

    --CO--R.sup.4

    --CO--CH.sub.2 --COR.sup.2

    --CH(OH)--COR.sup.2

    --CO--CF.sub.2 --COR.sup.2

    --CH(OH)--CF.sub.2 --COR.sup.2

    --CO--CO--NR.sup.5 R.sup.6

or

    --CO--CH.sub.2 --CO--NR.sup.5 R.sup.6

wherein all of the symbols are the same meaning as hereinbefore defined.

L² represents a group of the general formula:

    --CO--COR.sup.2

    --CH(OH)--COR.sup.2

    --CH(OR.sup.3).sub.2 ##STR19## or

    --CH(OH)--R.sup.4

wherein all of the symbols are the same meaning as hereinbefore defined.

A'represents a bond, alkylene group of from 1 to 6 carbon atom(s), alkenylene group of from 2 to 6 carbon atom(s), or a group of the general formula: ##STR20## wherein Y represents alkylene group of from 1 to 4 carbon atom(s) or alkenylene group of from 2 to 4 carbon atoms.

a saturated hydrocarbon ring of from 4 to 7 carbon atoms or heterocyclic mono ring containing two hetero atoms.

E' represents alkylene group of from 1 to 8 carbon atom(s), alkylene group of from 1 to 8 carbon atom(s) substituted by phenyl or benzyl group.

Alk' represents alkylene group of from 4 to 20 carbon atoms.

In the general formula (I) and (Id), "alkylene group of from 1 to 6 carbon atom(s)" represented by A, A' and B means methylene, ethylene, trimethylene, tetramethylene, pentamethylene and hexamethylene groups and isomers thereof.

In the general formula (I), (Id) and (Ie), "alkylene group from 1 to 8 carbon atom(s)" represented by E and E' means groups described above including heptamethylene and octamethylene groups and isomers thereof.

In the general formula (I), "alkylene group of from 1 to 4 carbon atom(s)" represented by Y means methylene, ethylene, trimethylene and tetramethylene group and isomers thereof.

In the general formula (I), "alkenylene group of from 2 to 4 carbon atoms" represented by Y means vinylene, propenylene, butenylene and butadienylene groups and isomers thereof.

In the general formula (I) and (Id), "alkenylene group of from 2 to 6 carbon atoms" represented by A and A' means groups described above including pentenylene, pentadienylene, hexenylene, hexadienylene and hexatrienylene groups and isomers thereof.

In the general formula (I) and (Id), "saturated hydrocarbon ring of from 4 to 7 carbon atoms" represented by A and A' means cyclobutane, cyclopentane, cyclohexane and cycloheptane.

In the general formula (I), heterocyclic mono ring represented by A means aromatic rings of from 3 to 7 ring members and wherein 1 or 2 hetero atom(s), which may be partially or fully saturated.

Examples of the rings mentioned above are furan, thiophene, pyrrole, oxazole, isoxazole, thiazole, isothiazole, imidazole, pyrazole, furazane, pyran, pyridine, pyridazine, pyrimidine, pyrazine rings and partially or fully saturated rings thereof.

In the general formula (I), preferred heterocyclic rings represented by A especially are piperidine, pyrrolidine and thiazolidine rings.

In the general formula.(Id), heterocyclic mono ring containing two hetero atoms means aromatic rings containing from 3 to 7 ring members which may be partially or fully saturated.

Examples of the rings mentioned above are oxazole, isoxazole, thiazole, isothiazole, imidazole, pyrazole, furazone, pyridazine, pyrimidine, pyrazine rings and partially or fully saturated rings thereof.

In the general formula (I), preferred heterocyclic ring represented by A' especially is thiazolidine ring.

In the general formula (If), "alkylene group of from 4 to 20 carbon atoms" represented by Alk' means tetramethylene, pentamethylene, hexamethylene, heptamethylene, octamethylene, nonamethylene, decamethylene, undecamethylene, dodecamethylene, tridecamethylene, tetradecamethylene, pentadecamethylene, hexadecamethylene, heptadecamethylene, octadecamethylene, nonadecamethylene, eicosamethylene groups and isomers thereof.

In the general formula (I), "alkylene group of from 1 to 3 carbon atom(s)" represented by Alk means methylene, ethylene, trimethylene group and isomers thereof.

In the general formula (I) "alkyl group of from 1 to 6 carbon atom(s)" represented by R¹, R², R³, R⁴, R⁵ and R⁶ means methyl, ethyl, propyl, butyl, pentyl and hexyl groups and isomers thereof.

In the general formula (I), "alkyl gorup of from 1 to 6 carbon atom(s)" in G and G' means the same as above.

In the general formula (I), "alkoxy group of from 1 to 6 carbon atom(s)" in R², G and G' means methoxy, ethoxy, propoxy, butoxy, penthyloxy and hexyloxy groups and isomers thereof.

In the general formula (I), "halogen atom" in G and G' means chlorine, bromine, fluorine and iodine atoms.

In the general formula (I), "carbocyclic ring" represented by G means mono-, di- or tri-cyclic aromatic carbocyclic ring(s) containing not more than 15 carbon atoms which may be partially or fully saturated.

Examples of the rings mentioned above are benzene, naphthalene, indene, azulene, fluorene, phenanthrene, anthracene, acenaphthalene, biphenylene rings and partially or fully saturated rings thereof.

In the general formula (I), "heterocyclic ring" represented by G means mono-, di- or tri-aromatic heterocyclic ring(s) containing not more than 15 carbon and hetero atoms which may be partially or fully saturated. In above heterocyclic rings, rings containing one or two of hetero atom(s) are preferred.

Examples of the rings mentioned above are furan, thiophene, pyrrole, oxazole, isoxazole, thiazole, isothiazole, imidazole, pyrazole, furazan, pyran, pyridine, pyridazine, pyrimidine, pyrazine, indole, isoindole, benzofuran, benzothiophene, indolizine, chromene, quinoline, isoquinoline, quinolizine, purine, indazole, quinazoline, cinnoline, quinoxaline, phthalazine, pteridine, carbazole, acridine, phenanthridine, xanthene, phenazine, phenothiazine rings and partially or fully saturated rings thereof.

In the general formula (I), preferred ring represented by G especially are benzene, naphthalene, fluorene, pyridine, furan, isoquinoline and acridine rings and partially saturated rings thereof.

In the above rings, substituted benzene rings are preferred as substituted rings by substituent(s).

In the present invention, hetetro atom(s) in the heterocyclic ring(s) means nitrogen, oxygen and sulfur atoms.

Throughout the specification including the claims, stereo isomers generated by stereo configuration(s) (asymmetric carbon, double bond etc.) and structural isomers generated by branching of a carbon chain, etc., are included in the present invention.

For example, it may be easily understood that alkylene and alkenylene groups include straight-chained and also branched-chained ones, to the skilled in the art.

Rings represented by A or rings in G may be attached to the adjacent group at any position.

NON-TOXIC SALTS

Among the compounds of the present invention of the general formula (I), compounds wherein L contains carboxy group may be converted into the corresponding salts. Non-toxic and water-soluble salts are preferable. Suitable salts, for example, are followings:

salts of alkaline metals (sodium, potassium, etc.),

salts of alkaline earth metal (calcium, magnesiums, etc.),

ammonium salts, salts of pharmaceutically acceptable organic amines (tetramethylammonium, triethylamine, methylamine, dimethylamine, cyclopentylamine, benzylamine, phenethylamine, piperidine, monoethanolamine, diethanolamine, tris(hydroxymethyl)amine, lysine, arginine, N-methyl-D-glucamine, etc.). The present invention also includes hydrate.

PROCESS FOR THE PREPARATION OF THE COMPOUNDS OF THE PRESENT INVENTION (1).

Among the compounds of the present invention of the general formula (I) compounds of the general formula: ##STR21## wherein, L²¹ represents a group of general formula:

    --CO--COR.sup.2, --CH(OH)--COR.sup.2 or --CO--CONR.sup.5 R.sup.6

wherein, all of the symbols have the same meaning as hereinbefore described, and the other symbols are the same meaning as hereinbefore defined,

may be prepared by processes described in the following reaction scheme [A].

Symbols in each formula represent the following meaning or as hereinbefore defined.

R²¹ : alkoxy group of from 1 to 6 carbon atom(s) ##STR22##

All of the reactions in the scheme [A] are known reactions; brief descriptions are the following:

Step <a> is N-acylation, and it may be carried out, for example, using benzoyl chloride and an aqueous solution of alkali (sodium bicarbonate, etc.) in a water-miscible organic solivent (acetone etc.).

Step <b> is oxidation, and it may be carried out, for example, by method of Swern oxidation, or Jones' oxidation.

Step <c> is hydrocyanation, and it may be carried out, for example, using sodium cyanate, in the presence of an acid (hydrochloric acid, etc.), in a water-miscible organic solvent (THF, etc.).

Step <d> is hydrolysis, and it may be carried out, for example, by refluxing in the presence of an acid (hydrochloric acid, etc.).

Step <e> is esterification, and it may be carried out, for example, using thionyl chloride or hydrochloride gas in a corresponding alcohol (methanol, ethanol, etc.).

Step <f> is a reaction to form amide, and it may be carried out, for example, to convert a carboxylic acid of the general formula (VIII) into a corresponding acid halide using an acid halogenating agent (thionyl chloride, oxalyl chloride, etc.), and the acid halide obtained was reacted with an amine of the general formula (VII) in the presence of a tertiary amine (triethylamine, etc.) in an inert organic solvent (methylene chloride, etc.); or to convert a carboxylic acid of the general formula (VIII) into an activated ester using a mixed acid anhydride (isobutyl chloroformate, ethylchloroformate, pivaloyl chloroformate, etc.). and the ester obtained was reacted with an amine of the general formula (VII) in the presence or absence of a tertiary amine (same as above).

Step <g> is oxidation, and it may be carried out, for example, by the method of Swern oxidation or Jones' oxidation.

Step <h> is hydrolysis, and it may be carried out, for example, using an aqueous solution of alkali (potassium carbonate, sodium hydroxide, etc.) in a water-miscible organic solvent (methanol, etc.).

Step <i> is a reaction to form amide, and it may be carried out, for example, using a chloroformic acid ester (isobutyl chloroformate, etc.) and a corresponding amine in the pressence of a tertiary amine.

Step <j> is esterification, and it may be carried out, for example, using a halogenating agent (benzyl bromide, alkyl bromides, etc.), in the presence of a base (potassium carbonate, triethylamine, etc.), in an inert organic solvent (DMSO, DMF, acetone, etc.).

Step <k> is hydrolysis, and it may be carried out by the procedure as described in step <h>.

Step <l> is esterification, ad it may be carried out by the same procedure as described in step <j>.

Step <m> is oxidation, and it may be carried out by the same procedure as described in step <g>.

PROCESS FOR THE PREPARATION OF THE COMPOUNDS OF THE PRESENT INVENTION (2)

Among the compounds of the present invention of the general formula (I), compounds of general formula: ##STR23## wherein, L²² represents

    --CH(OR.sup.3).sub.2, ##STR24##

    --CH(OH)--R.sup.4, --CO--R.sup.4, --CO--CH.sub.2 --COR.sup.2,

    --CH(OH)--CH.sub.2 --COR.sup.2, --CO--CF.sub.2 --COR.sup.2,

    --CH(OH)--CF.sub.2 --COR.sup.2

or

    --CO--CH.sub.2 --CO--NR.sup.5 R.sup.6,

and

the other symbols are the same meaning as hereinbefore defined,

may be prepared by processes described tn the following reaction scheme [B] or [C].

Symbols in each formula represent the following meaning or are as hereinbefore defined.

R⁴¹ : alkyl group of from 1 to 6 carbon atom(s), phenyl group, or alkyl group substituted by phenyl group, ##STR25##

All of the reactions in scheme [B] and [C] are known reactions and brief descriptions are the following:.

Step <n> is Reformatskij reaction, and it may be carried out, for example, by refluxing with a corresponding alkyl bromoacetate, in the presence of zinc powder and iodine, in an inert organic solvent (THF, benzene, etc.).

Step <o> is hydrolysis, and it may be carried out by the same procedure as discribed in step <h>.

Step <p> is mild-oxidation, and it may be carried out, for example, using Dess-Martin reagent, in an inert organic solvent (methylene chloride, etc.).

Step <q> is hydrolysis, and it may be carried out by the same procedure as described in step <h>.

Step <r> is a reaction to form amide, and it may be carried out by the same procedure as described in step <i>.

Step <s> is Reformatskij reaction and it may be carried out by the same procedure as described in step <n>, using a corresponding alkyl bromodifluoroacetate instead of alkyl bromoacetate.

Step <t> is hydrolysis, and it may be carried out by the same procedure as described in step <h>.

Step <u> is mild-oxidation, and it may be carried out by the same procedure as described in step <p>.

Step <v> is a hydrolysis, and it may be carried out by the same procedure as described in step <h>.

Step <w> is a reaction to form acetal, and it may be carried out, for example, using acid catalyst (camphorsulphonic acid, p-toluenesulphonic acid, etc.) in an alcohol (methanol, ethanol, etc.).

Step <x> is a reaction to form acetal, and it may be carried out, for example, by refluxing with ethylene glycol, in the presence of an acid catalyst (p-toluenesulphonic acid, etc.), in an inert organic solvent (benzene, etc.).

Step <y> is Grignard reaction, and it may be carried out, for example, using a corresponding Grignard reagent (alkylmagnesium chloride, etc.) in an ether (THF, etc.).

Step <Z> is oxidation, and it may be carried out by the same procedure as described in step <g>.

Step <aa> is fluoroalkylation, and it may be carried out, for example, using trifluoromethyl iodide under the irradiation of ultrasonic waves.

Step <bb> is oxidation, and it may be carried out by the same procedure as described is step <u>.

Starting materials and reagents in the present invention are known per se. For example, the compounds of the general formula (VIII) and (IX) are described with processes for the preparation in European Patent Publication No. 268190, 277588 or 280956 or Japanese Patent Publication No. 63-162672 or 63-264454.

Throughout the specification, in each reaction, products may be purified by conventional methods, for example, distillation at atmospheric or reduced pressure, high performance liquid chromatography, thin layer chromatography using silica gel or magnesium silicate or washing or recrystallization. Purification may be carried out after each reactions or a series of reactions.

PHARMACOLOGICAL ACTIVITIES

The compounds of the present invention of the general formula (I) possess an inhibitory activity on prolyl endopeptidase, described as before. For example, in a standard laboratory test, results in the following were given.

Prolyl Endopeptidase Inhibitory Activity in vitro

The compounds of the present invention showed activites as in the following Table I, with the test system described hereafter.

                  TABLE I                                                          ______________________________________                                                        Concentration for                                               Example No. of 50% inhibition                                                  the compounds  IC.sub.50 (μM)                                               ______________________________________                                         2                  0.003                                                       2          (b)     0.0018                                                      2          (d)     0.00085                                                     2          (g)     0.0017                                                      2          (h)     0.017                                                       2          (l)     0.0018                                                      6                  3.0                                                         7                  0.0012                                                      9                  0.023                                                       12                 69                                                          13                 0.10                                                        15                 1.5                                                         15         (a)     0.12                                                        17         (a)     0.18                                                        19                 14                                                          ______________________________________                                    

Inhibitory activity of prolyl endopeptidase in vitro was measured by the following test system.

A mixed solution of 20 mM tris-HCl buffer (pH 7.5; 935 μl; containing 10 mM EDTA and 10 mM mercaptoethanol), a solution of a compound of the present invention in DMSO (10 μl) and a solution of prolyl endopeptidase which was purified from bovine brain (0.13 unit ml; prepared by the method described in J. Biochem., 94, 1179 (1983)) in tris-HCl buffer (15 μl) was preincubated for 15 mins at 37° C.

To the solution, 5 mM of N-benzyloxycarbonyl-glycyl-prolyl-p-nitroanilide (40 μl) in a mixture of 40% dioxane--60% water was added. The solution was incubated for 1 min at the same temperature.

Optical absorption (a₁) at 405 nm of the solution, and optical absorption (a₂) at 405 nm of the solution after 30 mins' more incubation at 37° C. were measured.

Optical absorptions (b₁ and b₂) of the solutions using DMSO instead of the solution of the compound of the present invention were also measured.

Inhibitory ratio was calculated by the following expression and IC₅₀ (required concentration for 50% inhibition) was obtained (See Protein, Nucleic acid and Enzyme 25(6), 513, 1980.). ##EQU2##

TOXICITY

On the other hand, it was confirmed that the toxicity of the compounds of the present invention were very low. Therefore, the compounds of the present invention may be considered to be sufficiently safe and suitable for pharmaceutical use.

APPLICATION FOR PHARMACEUTICALS

To inhibit prolyl endopeptidase is to suppress the metabolism of neurotransmitters, substances taking part in memory in the brain (each of them is peptide) described hereinbefore, and therefore should be useful for prevention and/or treatment of amnesia, in animals including human beings, especially human beings.

The compounds of the present invention possess an inhibitory activity on prolyl endopeptidase in vitro, so they are expected to be useful for prevention and/or treatment of amnesia.

For the purpose above described, the compounds of the present invention may normally by administered systemically or locally, usually by oral or parenteral administration.

The doses to be administered are determined depending upon age, body weight, symptom, the desired therapeutic effect, the route of administration, and the duration of the treatment, etc. In the human adult, the doses per person per dose are generally between 1 mg and 500 mg, by oral administration, up to several times per day, and between 1 mg and 100 mg, by parenteral administration (preferably, intravenous administration) up to several times per day.

As mentioned above, the doses to be used depend upon various conditions, Therefore, there are cases in which doses lower than or greater than the ranges specified above may be used.

At the administration, the compounds of the present invention may be formed into solid compositions, liquid compositions or the other compositions for oral administration, injection compositions, external composition, suppositories, etc. for parenteral administration.

Solid compositions for oral administration include compressed tablets, pills, capsules, dispersible powders; and granules. In such solid compositions, one or more of the active compound(s) is or are admixed with at least one inert diluent (lactose, mannitol, glucose, hydroxypropylcellulose, micrycrystalline cellulose, starch, polyvinylpyrrolidone, magnesium metasilicate aluminate etc.).

The compositions may also comprise, as is normal practice, additional substances other than inert diluents: e.g., lubricating agents (magnesium stearate, etc.), disintegrating agents (cellulose calcium gluconate, etc.), and assisting agents for dissolving (glutamic acid, aspartic acid, etc.) and stabilizing agents (lactose, etc.).

The tablets or pills may, if desired, be coated with a film of gastric or enteric material (sugar, gelatin, hydroxypropylcellulose or hydroxypropylmethyl cellulose phthalate, etc.).

Capsules include soft ones and hard ones.

Liquid compositions for oral administration include pharmaceutically-acceptable solutions, emulsions, suspensions, syrups and elixirs.

In such liquid compositions, one or more of the active compound(s) is or are used in inert diluent(s) commonly used in the art (purified water, ethanol, etc.).

Besides inert diluents, such compositions may also comprise adjuvants (wetting agents, suspending agents, etc.), sweetening agents, flavouring agents, perfuming agents and preserving agents.

Other compositions for oral administration include spray compositions which may be prepared by known methods and which comprise one or more of the active compound(s).

Spray compositions may comprise additional substances other than inert diluents: e.g., stabilizing agents (sodium sulfite, etc.), isotonic buffer (sodium chloride, sodium citrate, citric acid, etc.).

For preparation of such spray compositions, for example, the method described in the U.S. Pat. Nos. 2,868,691 or 3,095,355 may be used.

Injections for parenteral administration include sterile aqueous or non-aqueous solutions, suspensions and emulsions. In such compositions, one or more of active compound(s) is or are admixed with at least one of inert aqueous diluent(s) (distilled water for injection, physiological salt solution, etc.) or inert non-aqueous diluent(s) (propylene glycol, polyethylene glycol, olive oil, ethanol, POLYSORBATE 80 (registered trade mark) etc.).

Injections may comprise other than inert diluents: e.g. preserving agents, wetting agents, emulsifying agents, dispersing agents, stabilizing agents (lactose, etc.), assisting agents such as assisting agents for dissolving (glutamic acid, aspartic acid, etc.).

They may be sterilized, for example, by filtration through a bacteria-retaining filter, by incorporation of sterilizing agents in the compositions or by irradiation. They may also be manufactured in the form of sterile solid compositions, for example, by freeze-drying, which can be dissolved in sterile water or some other sterile diluents for injection immediately before use.

Other compositions for parenteral administration include liquids for external use, and endermic liniments (ointments, etc.), suppositories and pessaries which comprise one or more of the active compound(s) and which may be prepared by known methods.

REFERENCE EXAMPLES AND EXAMPLES

The following reference examples and examples are to illustrate the present invention, but not limit the present invention.

The solvents in the parentheses show the developing or eluting solvents and the ratios of the solvents used are by volume in chromatographic separations.

Unless otherwise specified, "IR" were measured by a liquid film method.

REFERENCE EXAMPLE 1 Synthesis of (2S)-1-benzoylpyrrolidine-2-methanol ##STR26##

Benzoyl chloride (18.5 ml) and an aqueous (50 ml) solution of sodium carbonate (17.0 g) were added dropwise to a solution of (S)-2-pyrrolidinemethanol (12.0 g) in a mixture of acetone (70 ml) and water (14 ml). After addition, the reaction mixture was stirred for 30 mins at room temperature. The reaction mixture was filtered. Water was added to the filtrate. The mixture was extracted with methylene chloride. The extract was washed, dried and evaporated. The residue was purified by column chromatography on silica-gel (hexane-EtOAc) to give the title compound (24.1 g) having the following physical data:

TLC: Rf 0.19 (EtOAc).

REFERENCE EXAMPLE 2 Synthesis of (2S)-1-benzoylpyrrolidin-2-al ##STR27##

A solution of DMSO (27.9 ml) in methylene chloride (50 ml) was added to a solution of oxalyl chloride (16.4 ml) in methylene chloride (300 ml) which was cooled to -70° C.

A solution of (2S)-1-benzoylpyrrolidine-2-methanol (24.1 g) in methylene chloride (120 ml) was added dropiwise to the solution at -70° C. The mixture was stirred for 30 mins. Triethylamine (89.5 ml) was added dropwise to the reaction solution at -70° C. The mixture was stirred for 50 mins at the same temperature, and then rised to 0° C. gradually. Water was added to the solution.

The mixture was extracted with methylene chloride. The extract was washed, dried, and evaporated. The residue was purified by column chromatography on silica gel (hexane-EtOAc) to give the title compound (23.7 g) having the following physical data:

TLC: RF 0.38(EtOAc)

REFERENCE EXAMPLE 3 Synthesis of (2RS)-2-[(2S)-1-benzoylpyrrolidine]-2-hydroxyacetonitrile ##STR28##

(2S)-1-benzoylpyrrolidin-2-al (23.7 g) was dissolved into a mixture of THF (200 ml) and water (135 ml). Sodium cyanate (8.36 g) was added to the solution.

Conc. hydrochloric acid (10 ml) was added to the mixture with ice-cooling. The reaction mixture was stirred for 1 hr. at the same temperature, and for 20 mins. at room temperature. Water was added to the reaction mixture. The mixture was extracted with methylene chloride. The extract was washed, dried and evaporated to give the title compound having the following physical data:

TLC: Rf 0.44 & 0.51 (EtOAc)

REFERENCE EXAMPLE 4 Synthesis of (2RS)-2-[(2S)-pyrrolidin-2-yl]-2-hydroxyacetic acid ##STR29##

Conc. hydrochloric acid (210 ml) was added to (2RS)-2-[(2S)-1-benzoylpyrrolidine]-2-hydroxyacetonitrile (21 mg). The mixture was refluxed for 1 hr.

The reaction solution was cooled to room temperature, and filtered. The filtrate was diluted with water. The solution was washed with ether. The water layer was absorbed onto an amberite column. The column was eluted with aqueous ammonia.

The eluented solution was evaporated. The concentrate was dissolved in water. The solution was treated with active carbon. The filtrate was evaporated. The residue was cooled to -10° C. to deposit crystals. The crystals were gathered to give the title compound (5.84 g) having the following physical data:

TLC: Rf 0.16 (EtOAc:AcOH:H₂ O=3:1:1)

REFERENCE EXAMPLE 5 Synthesis of (2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidine-2-methanol ##STR30##

Triethylamine (4.0 ml) and isobutyl chloroformate (1.86 ml) were added dropwise to a solution of 3-(N-benzyl-N-methylcarbamoyl) propionic acid (2.5 g) in THF (30 ml) cooled to -20° C. The mixture was stirred for 30 mins at the same temperature. Triethylamine (4 ml) was added to a solution of (S)-2-pyrrolidinemethanol (1.45 g) in THF (30 ml). To the solution cooled to -25° C., the above solution was added dropwise.

The mixture was stirred for 1 hr at the same temperature. After reaction, water was added to the reaction solution. The mixture was extracted with EtOAc. The extract was washed, dried and evaporated. The residue was purified by column chromatography on silica gel (CH₂ Cl₂ --CH₃ OH) to give the title compound (2.59g) having the following physical data:

TLC: Rf 0.24 (EtOAc:CH₃ OH=9:1)

REFERENCE EXAMPLE 6 Synthesis of (2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-al ##STR31##

By the same procedure described in reference example 2, using (2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidine-2-methanol (2.59 g), the title compound (2.37 g) having the following phisical data was given:

TLC: Rf 0.40 (EtOAc: CH₃ OH=9:1)

EXAMPLE 1 Synthesis of (2RS)-2-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl) propanoyl]pyrrolidin-2-yl]-2-hydroxyacetic acid ethyl ester ##STR32##

Triethylamine (3.5 ml) and isobutyl chloroformate (980 μl) were added to a solution of 3-(N-methyl-N-benzylcarbamoyl) propionic acid (1.33 g) in THF (16 ml). The mixture was stirred for 15 mins at -15° C. The solution was named solution A. (2RS)-2-[(2S)-pyrrolidin-2-yl)-2-hydroxyacetic acid (1.0 g) was dissolved in ethanol (25 ml). The solution was cooled to -20° C. Thionyl chloride (1.0 ml) was added dropwise to the cooled solution. The mixture was stirred for 2 hrs. at room temperature. Triethylamine (5 ml) was added dropwise to the solution cooled to -20° C. again. The solution was allowed to stand to rise to room temperature, and evaporated.

THF (26 ml) and triethylamine (1.15 ml) were added to the residue. To the solution cooled to -10° C., the above solution A was added dropwise. The mixture was stirred for 2 hrs at room temperature. Water was added to the reaction solution. The mixture was extracted with EtOAc. The extract was washed, dried and evaporated. The residue was purified by column chromatography on silica gel (hexane - EtOAc) to give the title compound (1.67 g) having the following physical data:

TLC: Rf 0.41 (CH₂ Cl₂ :CH₃ OH=9:1);

IR (CHCl₃ solution): ν 3470˜3350, 3000, 1730, 1630, 1440, 1250˜1210, 1120 cm⁻¹.

EXAMPLE 1(a)-1(n)

By the same procedure as is described in title compounds having the physical data shown in table II were given. ##STR33##

                                      TABLE II                                     __________________________________________________________________________     Exam-                                                                          ple                                                                            No. R                    Name            TLC          IR(νcm.sup.-1)        __________________________________________________________________________     1(a)                                                                               φ-(CH.sub.2).sub.9                                                                              2-[2-[(2S)-1-(10-phenyldecanoyl)                                                               Ref 0.34     3450˜3200,                                                               2900,                                             pyrrolidin-2-yl]-2-hydroxyacetic                                                               (hexane:EtOAc = 1:1)                                                                        2840, 1720, 1630,                                 acid ethyl ester             1610, 1430, 1250,                                                              1180, 1100               1(b)                                                                                ##STR34##           2-[(2S)-1-]3-]N-4- trifluoromethylphenylmethyl)-                               -phenylcarbamoyl]propanoyl] pyrrolidin-2-yl]-2-hy                              droxyacetic acid ethyl ester                                                                   Ref 0.14 (hexane:EtOAc 32                                                                   3450˜3300,                                                               2950, 1720,                                                                    1650˜1610,                                                               1590, 1490, 1420,                                                              1390, 1315, 1260,                                                              1110, 1060, 1010,                                                              690                      1(c)                                                                                ##STR35##           2-[(2S)-1-[3-[4-(4-phenylbutoxy) phenyl]propenoyl                              ]pyrrolidin-2-yl] -2-hydroxyacetic acid ethyl                                  ester           Rf 0.26 (hexane:EtOAc                                                                       2950, 1720, 1630,                                                              1580, 1500, 1410,                                                              1240, 1160, 820,                                                               690                      1(d)                                                                                ##STR36##           2-[(2)-1-(3-(N-(4- chlorophenylmethyl)carbamoyl]                               ropanoyl]pyrrolidin-2-yl]- 2-hydroxyacetic acid                                etyl eter       Rf 0.31 (CH.sub.2 Cl.sub.2                                                     :CH.sub.3 OH = 10:1)                                                                        3300,                                                                          2990˜2930,                                                               1730, 1630, 1610,                                                              155O˜1530,                                                               1490, 1440˜143                                                           0, 1240, 1200,                                                                 1095, 1015               1(e)                                                                                ##STR37##           2-[(2S)-1-[3-[N-(9-fluorenyl) carbamoyl]propanoyl                              ]pyrrolidin- 2-yl]-2-hydroxyacetic acid ethyl                                  ester           Rf 0.42 (CH.sub.2 Cl.sub.2                                                     :CH.sub.3 OH = 10:1)                                                                        3460, 2990, 1725,                                                              1655, 1630, 1500,                                                              1445, 1370,                                                                    1245˜1205,                                                               1115                     1(f)                                                                                ##STR38##           2-[(2S)-1-[(2RS)-2-isopropyl-7- phenylheptanoyl]p                              yrrolidin-2-yl]- 2-hydroxyacetic acid ethyl                                    ester           Rf 0.67 (hexane:EtOAc                                                                       3350, 1720, 1600,                                                              1420, 1100, 1020,                                                              740, 690                 1(g)                                                                                ##STR39##           2-[(2S)-1-[3-[N-(1-naphtyl)- methyl-N-methylcarba                              noyl] propanoyl]pyrrolidin-2-yl]-2- hydroxyacetic                               acid ethyl ester                                                                              Rf 0.33 (EtOAc:CH.sub.3 OH =                                                   19:1)        3350, 1720, 1620,                                                              1400, 1100, 790,                                                               670                      1(h)                                                                                ##STR40##           2-[(2S)-1-[(1RS,2R)-2-(3- phenylpropanoyl)                                     cyclopentanecarbonyl]pyrrolidin- 2-yl]-2-hydroxya                              cetic acid ethyl ester                                1(i)                                                                                ##STR41##           2-[(2S)-1-[(1RS,2R)- 2-(n-benzylcarbamoyl)                                     cyclopentanecarbonyl]pyrrolidin- 2-yl]-2-hydroxya                              cetic acid ethyl ester                                                                         Rf 0.20 (EtOAc:hexane                                                                       3300, 1720, 1600,                                                              1510, 1420, 1220,                                                              1090, 1010, 670          1(j)                                                                                ##STR42##           2-[(2S)-1-[3-[N-(2,4-dichloro- phenylmethyl)carba                              moyl] propanoyl]pyrrolidin-2-yl]-2- hydroxyacetic                               acid ethyl ester                                                                              Rf 0.35 (EtOAc:CH.sub.3 OH =                                                   19:1)        3300, 3050, 1710,                                                              1600, 1520, 1410,                                                              1240, 1180, 1090,                                                              1010, 810, 665           1(k)                                                                                ##STR43##           2-[(2S)-1-[(2RS)-2-isopropyl-6-(4- methoxyphenyl)                              hexanoyl] pyrrolidin-2-yl]-2-hydroxyacetic acid                                ethyl ester     Rf 0.31 (hexane:EtOAc                                                                       3300, 1720, 1600,                                                              1500, 1420, 1240,                                                              1100, 1020, 810,                                                               670                      1(l)                                                                                ##STR44##           2-[(2S)-1-[3-(N-benzyl-N-t- butylcarbamoyl)propan                              oyl] pyrrolidin-2-yl]-2-hydroxyacetic acid ethyl                               ester           Rf 0.49 (CH.sub.2 Cl.sub.2                                                     :CH.sub.3 OH = 10:1)                                                                        3480˜3370,                                                               2975, 1735,                                                                    1640˜1620,                                                               1410, 1360, 1190,                                                              1110, 1025, 750,                                                               710                      1(m)                                                                                ##STR45##           2-[(2S)-1-[3-(N-benzyl-N- phenylcarbamoyl)propano                              yl] pyrrolidin-2-yl]-2-hydroxyacetic acid ethyl                                ester           Rf 0.48 (CH.sub.2 Cl.sub.2                                                     :CH.sub.3 OH = 10:1)                                                                        3450˜3350,                                                               2980, 1735, 1635,                                                              1595, 1495, 1405,                                                              1265, 1200, 1025,                                                              780, 730, 700            1(n)                                                                                ##STR46##           2-[(2S)-1-(4-oxo-7- phenylheptanoyl)pyrrolidin-2-                              yl]- 2-hydroxyacetic acid ethyl                                                                Rf 0.29 (EtOAc:hexane                                                                       3400, 1700, 1610,                                                              1410, 1090, 1010                                                               740,                     __________________________________________________________________________                                                           670                 

EXAMPLE 2 Synthesis of 2-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-yl]-2-oxoacetic acid ethyl ester ##STR47##

DMSO (443 μl) was added dropwise to a solution of oxyalyl chloride (260 μl) in methylene chloride (10 ml) cooled to -78° C. The solution was stirred for 15 mins at the same temperature. A solution of (2RS)-2-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl]propanoyl]pyrrolidin-2-yl]-2-hydroxyacetic acid ethyl ester (700 mg) in methylene chloride (6 ml) was added dropwise to the solution at the same temperature. The mixture was stirred for 20 mins. Triethylamine (1.42 ml) was added dropwise to the solution. The solution was stirred for 10 mins at the same temperature, and for 30 mins. at 0° C.

Water was added to the reaction solution. The mixutre was extracted with EtOAc. The extract was washed, dried and evaporated. The residue was purified by column chromatography on silica gel (hexane - EtOAc) to give the title compound (627 mg) having the following physical data:

TLC: Rf 0.18 (EtOAc);

IR (CHCl₃ solution): ν 3000, 1725, 1630, 1440, 1205, 1050 cm⁻¹

EXAMPLE 2(a)-2(s)

By the same procedure as is described in example 2, title compounds having the physical data shown in table III were given. ##STR48##

                                      TABLE III                                    __________________________________________________________________________     Ex-                                                                            ample                                                 IR (.sup.ν                                                                  cm.sup.-1)               No. R                       Name            TLC       or                       __________________________________________________________________________                                                           mp                       2(a)                                                                                ##STR49##              2-[(2S)-1-[3-[N-(4- chlorophenylmethyl)carbamo                                 yl] propanoyl]pyrrolidin-2-yl]-2- oxoacetic                                    acid ethyl ester                                                                               Rf 0.18 (EtOAc)                                                                          3300, 3070, 2980,                                                              1720,                                                                          1640˜1615,                                                               1550˜1525,                                                               1490 1435, 1370,                                                               1260, 1130, 1090,                                                              1055, 1010               2(b)                                                                                ##STR50##              2-[(2S)-1-[3-[N-(9-fluoroenyl) carbamoyl]propa                                 noyl]pyrrolidin- 2-yl]-2-oxoacetic acid ethyl                                  ester           Rf 0.31 (EtOAc)                                                                          mp                                                                             142˜146.degree                                                           . C.                     2(c)                                                                               φ-(CH.sub.2).sub.9  2-[ (2S)-1-(10-phenyldecanoyl)                                                                 Rf 0.48   3000˜2800,                                     pyrrolidin-2-yl]-2-oxoacetic                                                                   (EtOAc:hexane                                                                            1740˜1710,                                     ethyl ester     1:1       1650˜1610,                                                               1430˜1400,                                                               1250,                                                                          1100, 1050, 740,                                                               690                      2(d)                                                                                ##STR51##              2-[(2S)-1-[3-[N-(4- trifluoromethylphenylmethy                                 l)-N- phenylcarbamoyl]propanoyl] pyrrolidin-2-                                 yl]-2-oxoacetic acid ethyl ester                                                               Rf 0.14 (EtOAc:hexane =                                                                  3000˜2850,                                                               1740˜1710,                                                               1650˜1600,                                                               1590, 1480,                                                                    1430˜1390,                                                               1250, 1100, 1010,                                                              690                      2(e)                                                                                ##STR52##              2-[(2S)-1-[3-[4-(4-phenylbutoxy) phenyl]propen                                 oyl]pyrrolidin-2-yl- 2-oxoacetic acid ethyl                                    ester           Rf 0.40 (EtOAc:hexane =                                                                  2940, 2850, 1720,                                                              1620, 1590, 1500,                                                              1420, 1300, 1240,                                                              1170, 1045, 820,                                                               740, 690                 2(f)                                                                                ##STR53##              2-[(2S)-1-[3-(N-benzyl-N-t- butylcarbamoyl)pro                                 panoyl] pyrrolidin-2-yl-]-2-oxoacetic acid                                     ethyl ester     Rf 0.48 (EtOAc)                                                                          2970, 1720, 1630,                                                              1395, 1355, 1310,                                                              1255, 1190, 1130,                                                              1050                     2(g)                                                                                ##STR54##              2-[(2S)-1-[3-(N-benzyl-N- phenylcarbamoyl)prop                                 anoyl] pyrrolidin-2-yl]-2-oxoacetic acid                                       ethyl ester     Rf 0.49 (EtOAc)                                                                          2965, 1720, 1630,                                                              1400, 1355, 1260,                                                              1195, 1130, 1050         2(h)                                                                                ##STR55##              2-[(2S)-1-[(2RS)-2-isopropyl-7- phenylheptanoy                                 l]pyrrolidin-2-yl-]- 2-oxoacetic acid ethyl                                    ester           Rf 0.44 & 0.40 (hexane:EtOAc                                                   = 7:3)    3000˜2900,                                                               2850, 1720, 1620,                                                              1430, 1260, 1050,                                                              740, 690                 2(i)                                                                                ##STR56##              2-[(2S)-1-[3-[N-(1-naphthyl) methyl-N-methylca                                 rbamoyl] propanoyl]pyrrolidin-2-yl-]-2-                                        oxoacetic acid ethyl ester                                                                     Rf 0.28  (EtOAc)                                                                         (CHCl.sub.3                                                                    solution) 2950,                                                                1715, 1625, 1430,                                                              1250˜1200,                                                               1040                     2(j)                                                                                ##STR57##              2-[(2S)-1-[(1RS, 2R)- 2-(3-phenylpropanoyl                                     ]cyclopentane- carbonyl]pyrrolidin- 2-yl]-2-ox                                 oacetic acid ethyl ester                                                                       Rf 0.74 (EtOAc)                                                                          2900, 2840, 1700,                                                              1620, 1430, 1260,                                                              1050, 740, 690           2(k)                                                                                ##STR58##              2-[(2S)-1-[(1RS, 2R)-2-(N- benzylcarbamoyl)                                    cyclopentanecarbonyl]pyrrolidin- 2-yl-]2-oxoac                                 etic acid ethyl ester                                                                          Rf 0.45 & 0.52 (EtOAc)                                                                   3300, 2950, 2840,                                                              1720,                                                                          1660˜1610,                                                               1530, 1440,                                                                    1260˜1240,                                                               1130, 1050               2(l)                                                                                ##STR59##              2-[(2S)-1-[3-[N-(2,4- dichlorophenyl-                                          methyl)carbamoyl] propanoyl]pyrrolidin-2-yl-]-                                 2- oxoacetic acid ethyl ester                                                                  Rf 0.26 (EtOAc)                                                                          (CHCl.sub.3                                                                    Solution) 3450,                                                                2950, 1720, 1650,                                                              1620, 1500, 1430,                                                              1260˜ 1200,                                                              1090, 1040               2(m)                                                                                ##STR60##              2-[(2S)-1-[(2RS)-2-isopropyl-6-(4- methoxy-                                    phenyl)hexanoyl]pyrrolidin- 2-yl]-2-oxoacetic                                  acid ethyl ester                                                                               Rf 0.55 & 0.62 (EtOAc:hexane                                                   = 1:1)    2940, 1720, 1620,                                                              1500, 1430,                                                                    1290˜1240                                                                1050˜1030,                                                               820                      2(n)                                                                                ##STR61##              2-[(2S)-1-[4-oxo-7- phenylheptanoyl)pyrrolidin                                 -2-yl]- 2-oxoacetic acid ethyl                                                                 Rf 0.60 (EtOAc:benzene =                                                                 1710, 1620, 1420,                                                              1250, 1040, 740,                                                               690                      2(o)                                                                                ##STR62##              2-[(2S)-1-[3-[N-(2-naphthyl) methy-N-methylcar                                 bamoyl] propanoyl]pyrrolidin-2-yl]-2-                                          oxoacetic acid ethyl ester                                                                     Rf 0.32 (EtOAc)                                                                          3000˜2900,                                                               1720˜1710,                                                               1620, 1420˜140                                                           0, 1250, 1100,                                                                 1040, 810, 740           2(p)                                                                                ##STR63##              2-[(2S)-1-[3-[N-(4- methylphenylmethyl)carbamo                                 yl] propanoyl]pyrrolidin-2-yl]-2- 2-oxoacetic                                  acid ethyl ester                                                                               Rf 0.54 (CH.sub.2 Cl.sub.2                                                     :CH.sub.3 OH                                                                             3300,                                                                          2980˜2910,                                                               1720, 1630, 1530,                                                              1440, 1260, 1050         2(q)                                                                                ##STR64##              2-[(2S)-1-[3-N-(4- chlorophenylmethyl)-N-                                      phenylcarbamoyl]propanoyl] pyrrolidin-2-yl]-2-                                 oxoacetic acid ethyl ester                                                                     Rf 0.31 (CH.sub.2 Cl.sub.2                                                     :CH.sub.3 OH                                                                             2980, 2870, 1725,                                                              1660˜1630,                                                               1595, 1490, 1425,                                                              1400, 1265, 1205,                                                              1135, 1090, 1055,                                                              1015, 800, 780,                                                                700                      2(r)                                                                                ##STR65##              2-[(2S)-1-[3-[N-(4- chlorophenylmethyl)-N-                                     methylcarbamoyl]propanoyl] pyrrolidin-2-yl]-2-                                 oxoacetic acid ethyl ester                                                                     Rf 0.24 (EtOAc)                                                                          2950, 2850, 1710,                                                              1620,                                                                          1480˜1400,                                                               1250,                                                                          1105˜1050,                                                               1000, 790                2(s)                                                                                ##STR66##              2-[(2S)-1-[6-(2-naphthyl)methyl- 4-oxohexanoyl                                 ]pyrrolidin-2-yl]- 2-oxoacetic acid ethyl                                      ester           Rf 0.44 (EtOAc:hexane =                                                                  1700, 1610, 1420,                                                              1250, 1050, 810,         __________________________________________________________________________                                                           740                 

EXAMPLE 3 Synthesis of 2-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-yl)-2-oxoacetic acid monohydrate ##STR67##

A mixture of 2-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl) propanoyl]pyrrolidin-2-yl]-2-oxoacetic acid ethyl ester (626 mg), potassium carbonate (277 mg), methanol (7 ml) and water (4 ml) was stirred for 1 hr. at room temperature. Water was added to the reaction solution. The mixture was extracted with EtOAc. The extract was washed, dried and evaporated to give the title compound (458 mg) having the following physical data:

TLC: Rf 0.42 (EtOAc:AcOH:H₂ O)=3:1:1);

IR (CHClhd 3 Solution): ν 3000, 1725, 1625, 1445 cm⁻¹

EXAMPLE 3(a)-3(r)

By the same procedure as is described in example 3, title compounds having the physical date shown in table IV were obtained. ##STR68##

                                      TABLE IV                                     __________________________________________________________________________     Exam-                                                                          ple                                                  IR (νcm.sup.-1)        No. R                      Name            TLC       or                        __________________________________________________________________________                                                          mp                        3(a)                                                                                ##STR69##             2-[(2S)-1-[3-(N-(4- chlorophenylmethyl)carbamoy                                l] propanoyl]pyrrolidin-2-yl]-2- oxoacetic                                     acid            Rf 0.35 (EtOAc:AcOH: H.sub.2                                                   O) = 3:1:1)                                                                              mp                                                                             160˜162°                                                           C.                       3(b)                                                                                ##STR70##             2-[(2S)-1-[3-[N-(9-fluorenyl) carbamoyl]propano                                yl]pyrrolidin- 2-yl]-2-oxoacetic                                                               Rf 0.44 (EtOAc:AcOH: H.sub.2                                                   O) = 3:1:1)                                                                              mp                                                                             212˜215°                                                           C.                       3(c)                                                                                ##STR71##             2-[(2S)-1-[3-[N-(4- trifluoromethylphenylmethyl                                )-N- phenylcarbamoyl]propanoyl] pyrrolidin-2-yl                                ]-2-oxoacetic acid                                                                             Rf 0.10 (CHCl.sub.3 :CH.sub.3                                                  OH = 9:1) 3400˜3300,                                                               2890˜2840,                                                               1720, 1630˜1600                                                          , 1590, 1490,                                                                  1440˜1400,                                                               1320, 1160, 1105,                                                              1060, 1010, 700           3(d)                                                                               φ-(CH.sub.2).sub.9 2-[(2S)-1-(10-phenyldecanoyl)                                                                  Rf 0.10   3000˜2840,                                                               1720,                                                pyrrolidin-2-yl]2-oxoacetic acid                                                               (CHCl.sub.3 :CH.sub.3 OH                                                                 1580, 1440, 1230,                                                    9:1)      1030, 690                 3(e)                                                                                ##STR72##             2-[(2S)-1-[3-[4-(4-phenylbutoxy) phenyl]propeno                                yl]pyrrolidin- 2-yl]-2-oxoacetic                                                               Rf 0.15 (CHCl.sub.3 :CH.sub.3                                                  OH = 4:1) 3000˜2800,                                                               1710, 1630, 1590,                                                              1500, 1420, 1240,                                                              1160, 810, 680            3(f)                                                                                ##STR73##             2-[(2S)-1-[3-(N-benzyl-N-t- butylcarbamoyl)prop                                anoyl] pyrrolidin-2-yl] -2-oxoacetic                                                           Rf 0.49 (EtOAc:AcOH: H.sub.2 O                                                 = 3:1:1)  (CHCl.sub.3                                                                    Solution) 2975,                                                                1725, 1630, 1445,                                                              1405, 1355, 1185          3(g)                                                                                ##STR74##             2-[(2S)-1-[3-(N-benzyl-N- phenylcarbamoyl)propa                                noyl] pyrrolidin-2-yl]-2-oxoacetic                                                             Rf 0.49 (EtOAc:AcOH: H.sub.2 O                                                 = 3:1:1)  (CHCl.sub.3                                                                    Solution) 2990,                                                                1725, 1635, 1595,                                                              1495, 1405                3(h)                                                                                ##STR75##             2-[(2S)-1-[(2RS)-2-isopropyl-7- phenylheptanoyl                                ]pyrrolidin-2-yl]- 2-oxoacetic                                                                 Rf 0.08 (CHCl.sub.3 :CH.sub.3                                                  OH = 9:1) (CHCl.sub.3                                                                    Solution) 3300˜                                                          2920, 2850, 1720,                                                              1590,                                                                          1420, 1320,1420.about                                                          .1200, 1040               3(i)                                                                                ##STR76##             2-[(2S)-1-[3-(N-(1-naphthyl) methyl-N-methylcar                                bamoyl] propanoyl]pyrrolidin-2-yl]-2- oxoacetic                                 acid           Rf 0.05  (CHCl.sub.3 :CH.sub.3                                                 OH = 9:1) (KBr tablet)                                                                   3300˜3000,                                                               2900, 1720,                                                                    1630˜1590,                                                               1430˜1400,                                                               1230 1030, 790, 760       3(j)                                                                                ##STR77##             2-[(2S)-1-[(1RS,2R)-2-(3- phenylpropanoyl)cyclo                                - pentanecarbonyl]pyrrolidin- 2-yl]-2-oxoacetic                                 acid           Rf 0.08 (CHCl.sub.3 :CH.sub.3                                                  OH = 9:1) (KBr tablet)                                                                   3500˜3400,                                                               2950, 1690, 1580,                                                              1440, 690                 3(k)                                                                                ##STR78##             2-[(2S)-1-[(1RS,2R)-2-(N- benzylcarbamoyl)cyclo                                - pentanecarbonyl]pyrrolidin-2- yl]-2-oxoacetic                                 acid           Rf 0.05 (CHCl.sub.3 :CH.sub.3                                                  OH = 9:1) (KBr tablet)                                                                   3500˜3300,                                                               2950, 1720, 1610,                                                              1440, 1240, 1040,                                                              690                       3(l)                                                                                ##STR79##             2-[(2S)-1-[3-[N-(2,4- dichlorophenylmethyl)carb                                a- moyl]propanoyl]pyrrolidin-2- yl]-2-oxoacetic                                 acid           Rf 0.05 (CHCl.sub.3 :CH.sub.3                                                  OH =  9:1)                                                                               (KBr tablet)                                                                   3500˜3300,                                                               2950, 2900, 1720,                                                              1620, 1440, 1250,                                                              1040, 820                 3(m)                                                                                ##STR80##             2-[(2S)-1-[(2RS)-2-isopropyl- 6-(4-methoxypheny                                l)hexanoyl]- pyrrolidin-2-yl]-2-oxoacetic                                                      Rf 0.10 (CHCl.sub.3 :CH.sub.3                                                  OH = 9:1) 3500˜3300,                                                               2900, 1740˜1720                                                          , 1600˜1580,                                                             1500, 1440, 1230,                                                              1020, 810, 730, 690       3(n)                                                                                ##STR81##             2-[(2S)-1-(4-oxo-7- phenylheptanoyl)pyrrolidin-                                2-yl]- 2-oxoacetic acid                                                                        Rf 0.16 (EtOAc:CH.sub.3 OH =                                                   4:1)      (KBr tablet) 3460,                                                             1700, 1570, 1400,                                                              1250, 1180, 730,                                                               690                       3(o)                                                                                ##STR82##             2-[(2S)-1-[3-[N-(2-naphthyl) methyl-N-methylcar                                bamoyl] propanyl]pyrrolidin-2-yl]-2- oxoacetic                                 acid            Rf 0.60 (EtOAc:AcOH: H.sub.2 O                                                 = 3:1:1)  (KBr tablet)                                                                   3500˜ 3400,                                                              2900, 1710, 1610,                                                              1430, 1230                3(p)                                                                                ##STR83##             2-[(2S)-1-[3-[N-(4- methylphenylmethyl)carbamoy                                l] propanoyl]pyrrolidin-2-yl]-2- oxoacetic                                     acid            Rf 0.50 (EtOAc:AcOH: H.sub.2 O                                                 = 3:1:1)  (CHCl.sub.3                                                                    Solution) 2990,                                                                2880, 1725, 1640,                                                              1595, 1490, 1400,                                                              1265, 1095, 1045,                                                              1015                      3(q)                                                                                ##STR84##             2-[(2S)-1-[3-[N-(4- chlorophenylmethyl)-N-                                     phenylcarbamoyl]propanoyl] pyrrolidin-2-yl]-2-o                                xoacetic acid   Rf 0.56 (EtOAc:AcOH: H.sub.2 O                                                 = 3:1:1)  (KBr tablet) 3300,                                                             2920, 1730, 1605,                                                              1560, 1460, 1110,                                                              1240, 1190, 1110,                                                              1060, 1035                3(r)                                                                                ##STR85##             2-[(2S)-1-[3-[N-(4- chlorophenylmethyl)-N-                                     methylcarbamoyl)propanoyl] pyrrolidin-2-yl]-2-o                                xoacetic acid   Rf 0.10 (CHCl.sub.3 :CH.sub. 3                                                 OH = 9:1) (CHCl.sub.3                                                                    Solution) 2950˜                                                          2840, 1710, 1620,                                                              1430,                     __________________________________________________________________________                                                          1390, 1080                    EXAMPLE 4

Synthesis of 2-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-yl]-2-oxo-N-ethylacetamide ##STR86##

A solution of 2 [(2S)-1-[3-(N-benzyl-N-methylcarbamoyl) propanoyl]pyrrolidin-2-yl]-2-oxoacetic acid (98 mg) in THF (3 ml) was cooled to -20° C. Triethylamine (188 μl) and isobutyl chloroformate (77 μl) were added dropwise to the solution. The mixture was stirred for 20 mins at the same temperature. An aq. solution of ethylamine (70% ; 200 μl) was added dropwise to the solution at the same temperature. The solution was stirred for 1 hr, while the temperature of the solution was raised to room temperature gradually. Water was added to the reaction solution. The mixture was extracted with EtOAc. The extract was washed, dried and evaporated. The residue was purified by column chromatography on silica gel (CH₂ Cl₂ --CH₃ OH) to give the title compound (62 mg) having the following physical data:

TLC: Rf 0.34 (EtOAc:AcOH:H₂ O=3:1:1);

IR: ν 3300, 2980, 2940, 2875, 1730, 1630, 1520, 1435, 1375, 1355, 1240, 1145, 1120, 1040 cm⁻¹

EXAMPLE 4(a)-4(b)

By the same procedure as is described in example 4, title compounds having the physicial data shown in Table V were obtained.

                                      TABLE V                                      __________________________________________________________________________      ##STR87##                                                                     Example No.                                                                           L         Name            TLC          IR (.sup.ν cm.sup.-1)         __________________________________________________________________________     4(a)                                                                                   ##STR88##                                                                                ##STR89##                                                                                      ##STR90##                                                                                   ##STR91##                       4(b)                                                                                   ##STR92##                                                                                ##STR93##                                                                                      ##STR94##                                                                                   ##STR95##                       __________________________________________________________________________

EXAMPLE 5 Synthesis of (2RS)-2-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl) propanoyl]pyrrolidin-2-yl]-2-hydroxyacetic acid monohydrate ##STR96##

By the same procedure as is described in example 3, using (2RS)-2-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-yl]-2-hydroxyacetic acid ethyl ester (670 mg), the title compound (517 mg) having the following physical data was obtained.

TLC: Rf 0.42 (EtOAc:AcOH:H₂ O=3:1:1);

IR (CHCl₃ solution): ν 3000, 1725, 1625, 1440 cm⁻¹.

EXAMPLE 6 Synthesis of (2RS)-2-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl )propanoyl]pyrrolidin-2-yl]-2-hydroxyacetic acid benzyl ester ##STR97##

A mixture of (2RS)-2-[(2S)-1-[3-(N-benzyl-N-methlcarbamoyl) propanoyl]pyrrolidin-2-yl]-2-hydroxyacetic acid (134 mg), potassium carbonate (64 mg), benzyl bromide (51 μl) and DMSO (1 ml) was stirred for 1 hr at room temperature. Water was added to the mixture. The mixture was extracted with a mixture of hexane and EtOAc (1:1). The extract was washed, dried and evaporated. The residue was purified by column chromatography on silica gel (hexane-EtOAc) to give the title compound (141 mg) having the following physical data:

TLC: Rf 0.46 (CH₂ Cl₂ :CH₃ OH=1:1);

IR (KBr tablet): ν 3400˜3340, 2950˜2920, 1735, 1625, 1440˜1410, 1240, 1185, 1120 cm⁻¹.

EXAMPLE 6(a)

By the same procedure as is described in example 6, title compound having the physical data shown in table VI was given.

                                      TABLE VI                                     __________________________________________________________________________     Example                                                                        No.  Formula              Name            TLC  IR (.sup.ν cm.sup.-1)        __________________________________________________________________________     6(a)                                                                                 ##STR98##                                                                                           ##STR99##                                                                                      ##STR100##                                                                          ##STR101##                     __________________________________________________________________________

EXAMPLE 7 Synthesis of 2-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-yl)-2-oxoacetic acid benzyl ester ##STR102##

By the same procedure as is described in example 2, using (2S)-2-[(2S)-1-[3-N-benzyl-N-methylcarbamoyl)propanoyl)pyrrolidin-2-yl]-2-hydroxyacetic acid benzyl ester (129 mg), the title compound (109 mg) having the following physical data was given:

TLC: Rf 0.56 (CH₂ Cl₂ :CH₃ OH=10:1);

IR: ν 2950˜2910, 1720, 1630, 1435, 1260, 1120, 1050 cm⁻¹

EXAMPLE 8 Synthesis of (1RS)-1-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-yl])-2,2,2-trifluoroethanol ##STR103##

Trifluoroiodomethane (2.3 g) was cooled to -78° C. with a trap. DMF (7 ml), (2S)-1 -[3-(N-benzyl-N-methycarbamoyl)propanoyl]pyrrolidin-2-al (710 mg) and zinc (1.0 g) were added thereto. And the mixture was treated with ultrasonic waves for 2 hrs. at room temperature. 1N hydrochloric acid was added to the reaction solution. The mixture was extracted with EtOAc. The extract was washed, dried, and evaporated. The residue was purified by column chromatography on silica gel (hexane - EtOAc) to give the title compound (217 mg) having the following physical data:

TLC: Rf 0.24 & 0.33 (EtOAc);

IR: ν 3300˜2250, 2920, 1735, 1690, 1640˜1610, 1440˜1400, 1375, 1265, 1160, 1120 cm⁻¹

EXAMPLE 9 Synthesis of 1-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-yl]-2,2,2-trifluoroethan-1-one ##STR104##

A mixture of (1RS)-1-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl) propanoyl]pyrrolidin-2-yl]-2,2,2-trifluoroethanol (103 mg), Dess-Martin reagent (435 mg) and methylene chloride (1.5 ml) was stirred for 3 hrs. at room temperature. After reaction, the raction solution was diluted with ether. The diluted solution was added dropwise to a saturated aq. solution of sodium bicarbonate (30) wherein sodium thiosulfate (3.0 g) was dissolved. The mixture was stirred for 10 mins at room temperature. The mixture was extracted with EtOAc. The extract was washed, dried and evaporated. The residue was purified by column chromatography on silica gel (hexane - EtOAc) to give the title compound (52 mg) having the following physical data:

TLC: Rf 0. 32 (EtOAc);

IR: ν 3270˜3150, 2920, 1730, 1630˜1600, 1445˜1405, 1260˜1240, 1160 cm⁻¹

EXAMPLE 10 Synthesis of (3RS)-3-[(2S)-1-[3N-benzyl-N-methylcarbamoyl]propanoyl]pyrrolidin-2-yl]-3-hydroxypropionic acid ethyl ester ##STR105##

Zinc powder (482 mg) and a small amount of iodine were added to a solution of ethyl bromoacetate (0.70 ml) in THF (6 ml). The mixture was refluxed for 30 mins. The solution was added dropwise to a solution of (2)-1-[3-(N-benzyl-N-methylcarbamoyl]propanoyl]pyrrolidin-1-al in benzene (10 ml). The mixture was refluxed for 30 mins. After reaction, 1N hydrochloric acid was added to the mixture. The mixture was extracted with EtOAc. The extract was washed, dried, and evaporated. The residue was purified by column chromatography on silica gel (hexane-EtOAc) to give the title compound (1.21 g) having the following physical data:

TLC: Rf 0.38 (CH₂ Cl₂ :CH₃ OH=10:1);

IR: ν 3450˜3350, 2980, 1730, 1630, 1440˜1410, 1375, 1270˜1240, 1180˜1150, 1110, 1030 cm⁻¹

EXAMPLE 11 Synthesis of 3-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-yl)-3-oxopropionic acid ethyl ester ##STR106##

A solution of (3RS)-3-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl) propionyl]pyrrolidin-2-yl]-3-hydroxypropionic acid ethyl ester (230 mg) in acetone (1 ml) was cooled to -30° C. Excess Jones' reagent was added dropwise to the solution. The mixture was stirred for 1 hr at the same temperature, and for 1 hr at -20° C., and for 1 hr at -10° C. Isopropyl alcohol was added to the reaction solution. Water was added to the mixture. The mixture was extracted with EtOAc. The extract was washed, dried, and evaporated. The residue was purified by column chromatography on silica gel (hexane-EtOAc) to give the title compound (98 mg) having the following physical data:

TLC: Rf 0.50 (CH₂ Cl₂ :CH₃ OH=10:1)

IR: ν 2980˜2920, 1710, 1630, 1430˜1400, 1360, 1305, 1255, 1020 cm⁻¹

EXAMPLE 12 Synthesis of (3RS)-3-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-yl]-3-hydroxy-2,2-difluoropropionic acid ethyl ester ##STR107##

A mixture of zinc powder (520 mg) and THF (8 ml) was refluxed. A solution of ethyl bromo difluoroacetate (815 μl) in THF (6ml) was added dropwise to the solution. The mixture was refluxed for 2 mins. A solution of (2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-al (800 mg) in THF (8 ml) was added dropwide to the solution. The mixture was refluxed for 10 mins. After cooling, a saturated aq. solution of sodium bicarbonate was added to the solution. The mixture was extracted with EtOAc. The extract was washed, dried and evaporated. The residue was purified by column chrdmatography on silica gel (hexaneEtOAc) to give the title compound (754 mg) having the following physical data:

TLC: Rf 0.21 (EtOAc);

IR: ν 3270˜3200, 3000˜2925, 1780˜1760, 1635˜1605, 1440˜1425, 1375, 1310 cm⁻¹

EXAMPLE 13 Synthesis of 3-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-yl]-3-oxo-2,2-difluoropropionic acid ethyl ester ##STR108##

A solution of (3RS)-3-[(2S)-l-[3-(N-benzyl-N-methylcarbamoyl) propanoyl]pyrrolidin-2-yl]-3-hydroxy-2,2-difluoropropionic acid ethyl ester (250 mg) in methylene chloride (1.5 ml) was added dropwise to a suspension of Dess-Martin reagent (448 mg) in methylene chloride (6 ml). The mixture was stirred for 3 hrs. The reaction mixture was diluted with ether. A saturated aq. solution of sodium bicarbonate (30 ml) wherein sodium thiosulfate (2.26 g) was dissolved was added to the diluted solution. The mixture was stirred for 10 mins, and extracted with EtOAc. The extract was washed, dried and evaporated. The residue was purified by column chromatography on silica gel (hexane - EtoAc) to give the title compound (238 mg) having the following physical data:

TLC: Rf 0.25 (EtOAc);

IR: ν 3000˜2930, 1765, 1630, 1435, 1370, 1200, 1125, 1085, 1015 cm⁻¹

EXAMPLE 14 Synthesis of 3-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl )propanoyl]pyrrolidin-2-yl)-3-oxo-2,2-difluoropropionic acid monohydrate ##STR109##

By the same procedure as is described in Example 3, using 3-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl]propanoyl]pyrrolidin-2-yl)-3-oxo-2,2-difluoropropionic acid ethyl ester, the title compound having the following physical data was given:

TLC: Rf 0.40 EtOAc AcOH:H₂ O=3:1:1);

IR (CHCl₃ Solution): ν 2990, 2920, 1755, 1625, 1440, 1120 cm⁻¹

EXAMPLE 15 Synthesis of (1RS)-1-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-yl]-propan-1-ol ##STR110##

A solution of (2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-al (700 mg) in THF (5 ml) was cooled to -25° C. A solution of ethyl magnesium chloride in THF (2M, 1.5 ml) was added dropwise to the solution. The mixture was stirred for 30 mins at the same temperature, and for 30 mins at 0° C. and further 1 hr. at room temperature. After reaction, a saturated aq. solution of ammonium chloride was added to the mixture. The oily layer separated was washed, dried and evaporated. The residue was purified by column chromatography on silica gel (EtOAc - CH₃ OH) to give the title compound (270 mg) having the following physical data:

TLC: Rf 0.47 (EtOAc: CH₃ OH=9:1));

IR: ν 3350, 1600, 1400, 1220, 1090, 720, 680 cm⁻¹

EXAMPLE 15(a)

By the same procedure as is described in example 15, the title compound having the physical data shown in table VII was given.

                                      TABLE VII                                    __________________________________________________________________________      ##STR111##                                                                    Example                                                                        No.  Formula      Name              TLC        IR (.sup.ν cm.sup.-1)        __________________________________________________________________________     15(a)                                                                                ##STR112##                                                                                  ##STR113##                                                                                       ##STR114##                                                                                ##STR115##                     __________________________________________________________________________

EXAMPLE 16 Synthesis of 1-[(2S)-1-[3-N-benzyl-N-methy carbamoyl)propanoyl]pyrrolidin-2-yl]propan-1-one ##STR116##

By the same procedure as is described in example 2, using (1RS)-1-[(2)-1-[3-N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-yl]propan-1-ol, the title compound having the following physical data was given:

TLC: Rf 0.49 (CH₂ Cl₂ :CH₃ OH=19:1);

IR: ν 1700, 1620, 1400, 1100, 720, 670 cm⁻¹

EXAMPLE 17 Synthesis of (1RS)-1-phenyl-1-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl) propanoyl]pyrrolidin-2-yl]methanol ##STR117##

A solution of (2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-al (200 mg) in THF (5 ml) was cooled to -25° C. A solution of phenyl magnesium bromide in THF (2M, 0.49 ml) was added dropwise to the solution. The reaction solution was stirred for 30 mins, at the same temperature, and for 30 mins at 0° C. A saturated aq. solution of ammonium chloride was added to the reaction solution. The mixture was extracted with EtOAc. The extract was washed, dried, and evaporated. The residue was purified by column chromatography on silica gel (EtOAc-CH₃ OH) to give the title compound (45 mg) having the following physical data:

TLC: Rf 0.47 (EtOAc:CH₃ OH=19:1)

IR: ν 3350, 1610, 1420, 1180, 1040, 670 cm⁻¹

EXAMPLE 17(a)-17(c)

By the same procedure as is described in example 17, title compounds having the physical data shown in table VIII were obtained.

                                      TABLE VII                                    __________________________________________________________________________      ##STR118##                                                                    Example                                                                        No.  Formula         Name              Formula    IR (.sup.ν cm.sup.-1)     __________________________________________________________________________     17(a)                                                                                ##STR119##                                                                                     ##STR120##                                                                                       ##STR121##                                                                                ##STR122##                  17(b)                                                                                ##STR123##                                                                                     ##STR124##                                                                                       ##STR125##                                                                                ##STR126##                  17(c)                                                                                ##STR127##                                                                                     ##STR128##                                                                                       ##STR129##                                                                                ##STR130##                  __________________________________________________________________________

EXAMPLE 18 Synthesis of [(2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-yl]dimethoxymethane ##STR131##

α-Camphorsulphonic acid (11.6 mg) was added to a solution of (2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-al (160 mg) in methanol (2 ml). The mixture was stirred for 3 hrs. at room temperature. After reaction, the mixture was extracted with EtOAc. The extract was washed, dried, and evaporated. The residue was purified by column chromatography on silica gel (EtOAc-CH₂ Cl₂ CH₃ OH) to give the title compound (156 mg) having the following physical data:

TLC: Rf 0.42 (EtOAc:CH₂ Cl₂ :CH₃ OH=95:1);

IR: ν 2900, 1620, 1400, 1180, 1110, 1050, 960, 730, 690 cm⁻¹

EXAMPLE 18 (a)-18(b)

By the same procedure as is described in example 18, title compounds having the physical data shown in table IX were obtained.

                                      TABLE IX                                     __________________________________________________________________________      ##STR132##                                                                    Example                                                                        No.  R            Name             TLC              IR (.sup.ν                                                                  cm.sup.-1)                 __________________________________________________________________________     18(a)                                                                                ##STR133##                                                                                  ##STR134##                                                                                      ##STR135##                                                                                      ##STR136##                18(b)                                                                               φ-(CH.sub.2).sub.9                                                                       ##STR137##                                                                                      ##STR138##                                                                                      ##STR139##                __________________________________________________________________________

EXAMPLE 19 Synthesis of 2-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl)propanoyl]pyrrolidin-2-yl]-1,3-dioxolane ##STR140##

Ethylene glycol (0.3 ml), p-toluenesulphonic acid (1 mg) were added to a solution of (2S)-1-[3-(N-benzyl-N-methylcarbamoyl) propanoyl]pyrrolidin-2-al (164 mg) in benzene (3 ml). The mixture was refluxed for 1 hr. The reaction solution was poured into a saturated aq. solution of sodium bicarbonate. The mixture was extracted with benzene. The extract was washed, dried, and evaporated. The residue was purified by column chromatography on silica gel (CH₃ OH-EtOAc-CH₂ Cl₂) to give the title compound (79 mg) having the following physical data:

TLC: Rf 0.35 (CH₃ OH:EtOAc:CH₂ Cl₂ =1:9:5));

IR: ν 2900, 2850, 1720, 1620, 1400, 1110, 1050, 720, 680 cm⁻¹

EXAMPLE 20 Synthesis of 3-[(2S)-1-[3-(N-benzyl-N-methylcarbamoyl) propanoyl]pyrrolidin-2-yl]-3-oxopropionic acid monohydrate ##STR141##

By the same procedure as is described in example 3, using the compound prepared in example 11, the title compound having the following physical data was obtained:

TLC: Rf 0.62 (EtOAc:AcOH:H₂ O=3:1:1);

IR (CHCl₃ Solution): ν 3000, 1725, 1630, 1440, 1410, 1250˜1210 cm⁻¹

FORMULATION EXAMPLE

The following components were admixed by a conventional method and punched out to obtain 100 tablets each containing 50 mg of active ingredient.

    ______________________________________                                         (2RS)-2-[(2S)-N-[3-(N-benzyl-N-methylcarbamoyl)                                                              5 g                                              propanoyl]pyrrolidin-2-yl]-2-hydroxyacetic acid                                ethyl ester                                                                    Cellulose calcium glycolate (disintegrating agent)                                                         0.2 g                                              Magnesium stearate (lubricating agent)                                                                     0.1 g                                              Microcrystaline cellulose   4.7 g                                              ______________________________________                                     

What is claimed is:
 1. A thiazoline derivative of the formula: ##STR142## wherein R represents the general formula:

    G--E--D--B--A

wherein A represents a linkage which contains no atom, an alkylene group of from 1 to 6 carbon atoms(s), an alkenylene group of from 2 to 6 carbon atoms, a group of the formula: ##STR143## wherein Y represents an alkylene group of from 1 to 4 carbon atom(s) or an alkenylene group of from 2 to 4 carbon atoms, a saturated hydrocarbon ring of from 4 to 7 carbon atoms or a heterocyclic mono ring containing 3 to 7 ring members including 1 or 2 hetero atom(s) selected from N, O and S atoms which may be partially or fully saturated or aromatic, B represents a linkage which contains no atom or an alkylene group of from 1 to 6 carbon atom(s), D represents a linkage which contains no atom, an oxygen atom, a carbonyl group or a group of the formula:

    --NR.sup.1 --CO--or --CO--NR.sup.1 --

wherein R¹ represents a hydrogen atom, an alkyl group of from 1 to 6 carbon atom(s), a phenyl group or a benzyl group, E represents a linkage which contains no atom, an alkylene group of from 1 to 8 carbon atom(s) or an alkylene group of from 1 to 8 carbon atom(s) substituted or by a phenyl or benzyl group, G represents a mono-, bi- or tri-carbocyclic ring(s) containing not more than 15 carbon atoms which may be partially or fully saturated or aromatic, or a mono-, bi- or tri-heterocyclic ring(s) containing not more than 15 ring members including carbon and 1 or 2 hetero atoms selected from N, O and S atoms which may be partially or fully saturated or aromatic wherein said carbocyclic or heterocyclic ring(s) represented by G is unsubstituted or substituted by 1-3 of an alkyl group of from 1 to 6 carbon atoms(s), an alkoxy group of from 1 to 6 carbon atom(s), a halogen atom, a trifluoromethyl group or a nitro group, L represents a --CO--⁴ group wherein R⁴ represents an alkyl group of from 1 to 6 carbon atom(s), a phenyl group, an alkyl group of from 1 to 6 carbon atom(s), substituted by a phenyl group or a trifluoromethyl group, with the proviso that compounds wherein both of A and B are a linkage which contains no atom are excluded, or a non-toxic salt or hydrate thereof.
 2. A pharmaceutical composition for treating amnesia which comprises, as an active ingredient, an effective amount of a thiazoline derivative of formula (Ib) depicted in claim 1 or a non-toxic salt or hydrate thereof and a pharmaceutically acceptable carrier and/or coating.
 3. A method for prevention and treatment of amnesia which comprises administration of a therapeutically effective amount of a thiazoline derivative of formula (Ib) depicted in claim 1 or a non-toxic salt or hydrate thereof. 